Southern, Northern, and Western Blot: What’s The Difference? The main difference between Southern, northern, and western blot techniques is the target molecules. While western blots target proteins in ...

Western bolting is a well-established method that is currently relied on in biology laboratories for determining the specific proteins and their abundance within a given sample of cells or tissue.

Verify that the antibody is definitely able to detect the antigen in the species by checking data sheets and reference material Potential Cause: Primary and secondary antibody mismatch Raise the ...