Inteins have also been called protein introns, by analogy with (RNA) introns. Intein splicing occurs post-translationally in a self-catalytic process. Here, the extein is shown in red and the intein in …

Here, we review the intein field, first focusing on the biological context and phylogenetic diversity of inteins, followed by a description of intein structure and biochemical function.

Mar 19, 2025 · The optimized CLm intein (Cysteine-Less and monomeric) retains the wild-type’s ultra-fast reaction rate and serves as an efficient, thiol-independent protein modification tool.

Feb 21, 2022 · The protein splicing of class 1 intein is achieved through structural conformational change and chemical bonds shifting on junction sites between intein and exteins, which can be …

An intein is a protein sequence embedded within a precursor protein sequence and excised during protein maturation, that is, post-translationally (Perler et al., 1994).

Aug 12, 2004 · The primary indication of intein origin lies in its two-domain structure, suggesting that a mobile intein is a result of a fusion between two proteins, most likely, a self-splicing …

Nov 8, 2023 · Protein splicing is a posttranslational process in which an intein segment excises itself from two flanking peptides, referred to as exteins. In the native context, protein splicing …

Inteins are mobile genetic elements that apply standard enzymatic strategies to excise themselves post-translationally from the precursor protein via protein splicing. Since their …

Intein-mediated protein splicing raises questions and creates opportunities in many scientific areas. Evolutionary biologists question whether inteins are primordial enzymes or simply …

Jul 1, 2024 · When an intein is introduced into a new host, the homing endonucleases target and disrupt conserved essential genes, such as polymerases, which the host cannot effectively …